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Part:BBa_K274004:Design

Designed by: Shuna Gould   Group: iGEM09_Cambridge   (2009-10-19)


Vio operon ABCE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1029
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4289
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3814
    Illegal AgeI site found at 4010
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The origonal vio operon was designed with the vioD gene flanked by complementary sticky end restriction sites for BglII and BclI. This allowed the vioD to be easily removed, leading to the production of the light green pigment.


Source

This part was made from the vio operon biobrick, part number BBa_K274002. The vioD gene has been removed by restriction digest.

References